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当前位置: 首页 > 产品中心 > Crushing_blending > PromoCell/Human M2 Macrophages (M-CSF) monocyte-derived
商品详细PromoCell/Human M2 Macrophages (M-CSF) monocyte-derived
PromoCell/Human M2 Macrophages (M-CSF) monocyte-derived
PromoCell/Human M2 Macrophages (M-CSF) monocyte-derived
商品编号: C-12915
品牌: PromoCell
市场价: ¥0.00
美元价: 0.00
产地: 美国(厂家直采)
公司:
产品分类: 破碎混匀
公司分类: Crushing_blending
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

PromoCell’s crypreserved human monocyte-derived macrophages (hMDM) are convenient and easy-to-handle. The thawed cells plate into all tissue culture vessel formats and can be maintained as adherent, biologically functional cultures for several weeks. Optionally, user-customizable activation of the cells can be performed.

Cryopreserved human macrophages are produced from monocytes in PromoCell’s well-proven M2 Macrophage Generation Media DXF and are available as non-activated, fully qualified M2- (M-CSF) polarized cells. After 9-10 days of differentiation, the M2 macrophages are cryopreserved using PromoCells’s proprietary, defined and animal-free freezing medium, Cryo-SFM. Each cryo vial contains more than 1.5 million or 5 million viable cells after thawing, respectively.

NEW: Our M2 Macrophages are now also available from HLA-typed donors.

Recommended plating density200,000 cells per cm2
Tested MarkersCD163 positive, CD68 positive
CryoMacrophages an Overview
Figure 1. Culture of PromoCell"s cryopreserved human macrophages. The macrophages can be directly used for experiments after thawing and plating. Optional: activation of polarized M1-/M2 macrophages 24h before assay.
CryoMacrophages an Overview
Figure 1. Culture of PromoCell"s cryopreserved human macrophages. The macrophages can be directly used for experiments after thawing and plating. Optional: activation of polarized M1-/M2 macrophages 24h before assay.
CryoMacrophages-FACS
Figure 2. Flow cytometric analysis of PromoCell cryopreserved Human M1 and M2 Macrophages. M1 macrophages exhibit a CD68+ (99,74%) and CD80+ (83,33%) marker expression profile, typical for M1 macrophages (upper row). M2 macrophages exhibit a CD68+ (99,72%) and CD163+ (94,91%) marker expression profile, typical for M2 macrophages (lower row).
CryoMacrophages-FACS
Figure 2. Flow cytometric analysis of PromoCell cryopreserved Human M1 and M2 Macrophages. M1 macrophages exhibit a CD68+ (99,74%) and CD80+ (83,33%) marker expression profile, typical for M1 macrophages (upper row). M2 macrophages exhibit a CD68+ (99,72%) and CD163+ (94,91%) marker expression profile, typical for M2 macrophages (lower row).
品牌介绍
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