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当前位置: 首页 > 产品中心 > Sequencing_enzyme > 促增殖细胞/人M2巨噬细胞(M-CSF)单核细胞衍生的
商品详细促增殖细胞/人M2巨噬细胞(M-CSF)单核细胞衍生的
促增殖细胞/人M2巨噬细胞(M-CSF)单核细胞衍生的
促增殖细胞/人M2巨噬细胞(M-CSF)单核细胞衍生的
商品编号: C-12917
品牌: PromoCell
市场价: ¥0.00
美元价: 0.00
产地: 美国(厂家直采)
公司:
产品分类: 测序酶
公司分类: Sequencing_enzyme
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

PromoCell’s crypreserved human monocyte-derived macrophages (hMDM) are convenient and easy-to-handle. The thawed cells plate into all tissue culture vessel formats and can be maintained as adherent, biologically functional cultures for several weeks. Optionally, user-customizable activation of the cells can be performed.

Cryopreserved human macrophages are produced from monocytes in PromoCell’s well-proven M2 Macrophage Generation Media DXF and are available as non-activated, fully qualified M2- (M-CSF) polarized cells. After 9-10 days of differentiation, the M2 macrophages are cryopreserved using PromoCells’s proprietary, defined and animal-free freezing medium, Cryo-SFM. Each cryo vial contains more than 1.5 million or 5 million viable cells after thawing, respectively.

NEW: Our M2 Macrophages are now also available from HLA-typed donors.

Recommended plating density200,000 cells per cm2
Tested MarkersCD163 positive, CD68 positive
CryoMacrophages an Overview
Figure 1. Culture of PromoCell"s cryopreserved human macrophages. The macrophages can be directly used for experiments after thawing and plating. Optional: activation of polarized M1-/M2 macrophages 24h before assay.
CryoMacrophages an Overview
Figure 1. Culture of PromoCell"s cryopreserved human macrophages. The macrophages can be directly used for experiments after thawing and plating. Optional: activation of polarized M1-/M2 macrophages 24h before assay.
CryoMacrophages-FACS
Figure 2. Flow cytometric analysis of PromoCell cryopreserved Human M1 and M2 Macrophages. M1 macrophages exhibit a CD68+ (99,74%) and CD80+ (83,33%) marker expression profile, typical for M1 macrophages (upper row). M2 macrophages exhibit a CD68+ (99,72%) and CD163+ (94,91%) marker expression profile, typical for M2 macrophages (lower row).
CryoMacrophages-FACS
Figure 2. Flow cytometric analysis of PromoCell cryopreserved Human M1 and M2 Macrophages. M1 macrophages exhibit a CD68+ (99,74%) and CD80+ (83,33%) marker expression profile, typical for M1 macrophages (upper row). M2 macrophages exhibit a CD68+ (99,72%) and CD163+ (94,91%) marker expression profile, typical for M2 macrophages (lower row).
品牌介绍
PromoCellPromoCell公司提供高质量的人体原代细胞、干细胞和血液细胞和癌细胞培养产品,以及细胞生物学产品包括抗体、细胞因子、生长因子等。科学和医学的进步是人类努力的动力。PromoCell支持您的生物医学研究。我们是原代细胞和细胞培养基以及细胞生物学研究的合作伙伴。我们的产品组合包括7,000多种遵循最严格的道德和质量标准的产品。产品列表:没有。品牌货号名称规格1PromoCellPK-EL-K21121,25-二羟基维生素D ELISA试剂盒,人类48项测试2PromoCellPK-AB913-1762K抗体(mAb)500微克3PromoCellPK-CA707-999025X Annexin V结合缓冲液15毫升4PromoCellPK-CA577-2338-1Akt抑制剂(10-NCP)1毫克5PromoCellPK-CA577-K131半胱天冬酶荧光底物组7 x 25测定6PromoCellPK-CA577-1727-1XAV9391毫克7PromoCellPK-CA707-400377-AAD(7-氨基放线菌素D)1毫克8PromoCellPK-CT-2003-005MA Lipofection Enhancer50微升9PromoCellPK-PF350LSS-2-01PromoFluor LSS氨基修饰标签1毫克10PromoCellPK-CA91-0117发酵支原体DNA标准品(用于qPCR)100微升11PromoCellC-12710人软骨细胞(HCH)50万个细胞12PromoCellC-28050树突状细胞生成培养基250毫升13PromoCellC-12810人心肌细胞(HCM)50万个细胞14PromoCellC-28040骨周生长培养基500毫升15PromoCellC-30020牛垂体提取物2毫升