The PromoCell Macrophage Generation Media have been developed for the efficient generation of monocyte-derived macrophages (MDM) from freshly isolated peripheral blood monocytes or directly from PBMC as a starting material. The M2-Macrophage Generation Medium DXF contains M-CSF and allows for the generation of M2-polarized macrophages. PromoCells M2-Macrophage Generation Medium DXF is a ready-to-use medium including cytokines for the directed differentiation of M2-like polarized MDM.

The Macrophage Media DXF are defined and xeno-free formulations for use with freshly isolated cells. Due to the utilization of exclusively synthetic, recombinant or plant-sourced materials, this medium is free of all animal-derived components and substances of human origin with human serum albumin as the only exception.

All media are optimized for primary human cells. However, we have received feedback from customers that this medium can also be used for murine macrophages.

To view a list of references where this medium was used with other species/cell types please click here.

Components:

• Basal Medium M2 (250ml)
• SupplementMix M2 (for 250ml)
• CytokineMix M2: M-CSF (for 250ml)