The PromoCell 3D Tumorsphere Medium XF is a culture system developed for the standardized serial culture of cancer cell lines as tumorspheres/mammospheres. The formulation supports sustained cell proliferation allowing for serial passage of the 3D culture. Its serum-free and xeno-free formulation provides a culture environment devoid of all stimuli originating from non-defined materials.
Components:
The PromoCell 3D Tumorsphere Medium XF consists of a bottle of Basal Medium and one vial of SupplementMix. Adding the SupplementMix to the Basal Medium results in the complete medium.
Key features:
- Establish 3D tumorsphere cultures directly from 2D cancer cell lines
- Suitable for long-term routine culture of tumorspheres
- Compatible with most commonly used cancer cell lines
- Xeno-free and serum-free formulation
List of cell types tested for serial passage with the PromoCell 3D Tumorsphere Medium XF:
| Tissue | Tested Cell Lines | Cell Lines Origin |
|---|
| Brain | U-87 MG | grade IV glioblastoma / astrocytoma of the human brain |
| Breast | MCF-7 | pleural effusion of metastatic human breast adenocarcinoma |
| Breast | MDA-MB-231 | pleural effusion of metastatic human breast adenocarcinoma (triple-negative) |
| Colon | HT-29 | human colon adenocarcinoma |
| Connective tissue | HT1080 | human fibrosarcoma |
| Liver | HepG2 | hepatocellular carcinoma of the human liver |
| Lung | A-549 | human lung carcinoma |
| Pancreas | Panc-1 | epithelioid carcinoma of the human pancreatic duct |
| Prostate | LNCaP | lymph node metastasis of human prostate adenocarcinoma |
| Skin | A-431 | epidermoid carcinoma of the human skin |
