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当前位置： 首页 > 产品中心 > Stimulus > 促进细胞/癌细胞系培养基XF
商品详细促进细胞/癌细胞系培养基XF
促进细胞/癌细胞系培养基XF
促进细胞/癌细胞系培养基XF
商品编号： C-28077
品牌： PromoCell
市场价： ¥0.00
美元价： 0.00
产地： 美国(厂家直采)
公司：
产品分类： 刺激/抑制试剂
公司分类： Stimulus
联系Q Q： 3392242852
电话号码： 4000-520-616
电子邮箱： info@ebiomall.com
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商品介绍

The PromoCell Cancer Cell Line Medium XF is a cell culture medium developed for the standardized in vitro cultivation of established human cancer cell lines. Its serum-free and xeno-free formulation provides a culture environment devoid of all stimuli originating from non-defined materials. Thus, it has no ill-defined components such as fetal calf serum, extracts or hydrolysates and exhibits very low lot-to-lot variability.

Components:The PromoCell Cancer Cell Line Medium XF consists of a bottle of Basal Medium and one vial of SupplementMix. Adding the SupplementMix to the Basal Medium results in the complete medium

Note: Culture vessels need to be coated. For coating we recommend using Human Fibronectin Solution (C-43060) or Vitronectin (C-69201).

Key features:

  • Suitable for long-term routine culture of adherently growing established human cancer cell lines
  • Compatible with most commonly used human cancer cell lines
  • Xeno-free and serum-free formulation

List of cell types tested for serial passage with the PromoCell Cancer Cell Line Medium XF:

TissueTested Cell LinesCell Lines OriginRemarks
BrainBV2immortalized murine primary microglial cellsCoat with Fibronectin:1 µg/cm2
BreastMCF-7pleural effusion of metastatic human breast adenocarcinomaCoat with Fibronectin:1 µg/cm2
ColonHT-29human colon adenocarcinomaCoat with Vitronectin:0.5 µg/cm2
Connective tissueHT1080human fibrosarcomaCoat with Fibronectin:1 µg/cm2
LiverHepG2hepatocellular carcinoma of the human liverCoat with Vitronectin:0.5 µg/cm2
LungA-549human lung carcinomaCoat with Vitronectin:0.5 µg/cm2
ProstateLNCaPlymph node metastasis of human prostate adenocarcinoma3D culture in C-28070 is recommended
Peripheral bloodMV-4-11Human acute myelogenous leukemia (suspension)No coating required
Bone marrowKG-1Human acute myelogenous leukemia (suspension)No coating required
KidneyACHNHuman Renal Cell CarcinomaCoat with Fibronectin:1 µg/cm2
BrainA172Human GlioblastomaCoat with Fibronectin:1 µg/cm2
Skeletal muscleC2C12Mouse MyoblastsCoat with Fibronectin:1 µg/cm2
SkinB16-F10Mouse MelanomaCoat with Fibronectin:1 µg/cm2
Abelson murine leukemia virus-induced tumorRAW264.7Mouse MacrophageCoat with Fibronectin:1 µg/cm2
Brain, neuroectodermalNeuro-2aMouse NeuroblastomaCoat with Fibronectin:1 µg/cm2
Figure 1. This figure shows example growth curves of four frequently used cancer cell lines cultivated in Cancer Cell Line Medium XF (C-28077), a serum- and xeno-free culture system. All of them exhibited a constant proliferation rate. The cell lines were plated at 5,000 cells/cm2 in T25 flasks coated with appropriate attachment substrates. For HT1080, 1 µg/cm2 of human fibronectin was used, while A549, HT29, and HepG2 were seeded on 0.5 µg/cm2 of recombinant human vitronectin. The cells were subcultured for between four and seven days depending on the cell line’s proliferation rate. The cumulative population doublings of 10 subsequent passages are shown for each cell line.
Figure 1. This figure shows example growth curves of four frequently used cancer cell lines cultivated in Cancer Cell Line Medium XF (C-28077), a serum- and xeno-free culture system. All of them exhibited a constant proliferation rate. The cell lines were plated at 5,000 cells/cm2 in T25 flasks coated with appropriate attachment substrates. For HT1080, 1 µg/cm2 of human fibronectin was used, while A549, HT29, and HepG2 were seeded on 0.5 µg/cm2 of recombinant human vitronectin. The cells were subcultured for between four and seven days depending on the cell line’s proliferation rate. The cumulative population doublings of 10 subsequent passages are shown for each cell line.
Figure 2. Phase-contrast images of various cell lines cultured in serum- and xeno-free Cancer Cell Line Medium XF (C-28077). The cell lines robustly maintained their typical morphological phenotypes while exhibiting faster proliferation rates that with conventional serum-containing culture systems. The magnifications of the images shown are A: 100x, B: 40x, C: 100x, D: 40x, E: 100x, and F: 100x.
Figure 2. Phase-contrast images of various cell lines cultured in serum- and xeno-free Cancer Cell Line Medium XF (C-28077). The cell lines robustly maintained their typical morphological phenotypes while exhibiting faster proliferation rates that with conventional serum-containing culture systems. The magnifications of the images shown are A: 100x, B: 40x, C: 100x, D: 40x, E: 100x, and F: 100x.
品牌介绍
PromoCellPromoCell公司提供高质量的人体原代细胞、干细胞和血液细胞和癌细胞培养产品，以及细胞生物学产品包括抗体、细胞因子、生长因子等。科学和医学的进步是人类努力的动力。PromoCell支持您的生物医学研究。我们是原代细胞和细胞培养基以及细胞生物学研究的合作伙伴。我们的产品组合包括7,000多种遵循最严格的道德和质量标准的产品。产品列表：没有。品牌货号名称规格1PromoCellPK-EL-K21121,25-二羟基维生素D ELISA试剂盒，人类48项测试2PromoCellPK-AB913-1762K抗体（mAb）500微克3PromoCellPK-CA707-999025X Annexin V结合缓冲液15毫升4PromoCellPK-CA577-2338-1Akt抑制剂（10-NCP）1毫克5PromoCellPK-CA577-K131半胱天冬酶荧光底物组7 x 25测定6PromoCellPK-CA577-1727-1XAV9391毫克7PromoCellPK-CA707-400377-AAD（7-氨基放线菌素D）1毫克8PromoCellPK-CT-2003-005MA Lipofection Enhancer50微升9PromoCellPK-PF350LSS-2-01PromoFluor LSS氨基修饰标签1毫克10PromoCellPK-CA91-0117发酵支原体DNA标准品（用于qPCR）100微升11PromoCellC-12710人软骨细胞（HCH）50万个细胞12PromoCellC-28050树突状细胞生成培养基250毫升13PromoCellC-12810人心肌细胞（HCM）50万个细胞14PromoCellC-28040骨周生长培养基500毫升15PromoCellC-30020牛垂体提取物2毫升
公司介绍
PromoCellPromoCell公司提供高质量的人体原代细胞、干细胞和血液细胞和癌细胞培养产品，以及细胞生物学产品包括抗体、细胞因子、生长因子等。科学和医学的进步是人类努力的动力。PromoCell支持您的生物医学研究。我们是原代细胞和细胞培养基以及细胞生物学研究的合作伙伴。我们的产品组合包括7,000多种遵循最严格的道德和质量标准的产品。产品列表：没有。品牌货号名称规格1PromoCellP
品牌分类
细胞生物学 人原代细胞培养 n. 促进( promotion的名词复数 );提升;推广;宣传; 癌细胞培养 人类茎 支原体安全
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